Separations of lipids by silver ion chromatography

Normal-phase separation effects with lipids on a silver Ion high- performance liquid chromatography column

The separation of saturated fatty acids (as the methyl esters and as triacylglycerols) of differing chain lengths by silver ion chromatography (ChromSpher Lipids: containing silver ions: Chrompack, Middelburg, Netherlands) was studied, The retention characteristics (isocratic solvent system of acetonitrile in hexane) of saturated fatty acid methyl esters and homogeneous, saturated triacylglycer...

Determination of trans octadecenoic acids by silver-ion chromatography-gas liquid chromatography: an intercomparison of methods.

Several silver-ion chromatography-gas liquid chromatography (GLC) techniques for the determination of trans octadecenoic acids in partially hydrogenated vegetable fats were collaboratively evaluated. Twelve laboratories participated in the study. All collaborators used high polarity fused silica capillary columns for the separation of fatty acid methyl esters by GLC; 7 collaborators isolated tr...

Chiral Separations: Liquid Chromatography

The use of CE in the analysis of pharmaceuticals is now becoming Rrmly established as a useful complement and alternative to the more widely employed technique of HPLC. The major attractions of CE are that considerable cost and time savings are possible, especially in the areas of chiral analysis and in the determination of solutes having limited or no chromophore. The recent advent of the use ...

Analytical separations of metals by column chromatography on microcrystalline cellulose

Optimization of azeotropic protein separations in gradient and isocratic ion-exchange simulated moving bed chromatography.

The separation of dilute binary mixtures of proteins by salt aided ion-exchange simulated moving bed (SMB) chromatography is optimized with respect to throughput, desorbent consumption and salt consumption. The optimal flow-rate ratios are analytically determined via an adopted "triangle theory". Azeotropic phenomena are included in this procedure. The salt concentrations in the feed and recycl...